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KMID : 1094720100150040651
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 4 p.651 ~ p.656
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Dissolved-oxygen-stat fed-batch fermentation of 1,3-dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112
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Hu Zhong-Ce
Zheng Yu-Guo
Shen Yin-Chu
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Abstract
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This study investigated the effects of DO concentration on DHA fermentation and of DO-stat fed-batch fermentation using a pH control strategy, on 1,3-dihydroxyacetone (DHA) production. The results showed that DO-stat fed-batch fermentation with pH-shift control was the optimal bioprocess for DHA production. DO-stat fed-batch fermentation was carried out at 30% air saturation, and the culture pH was automatically maintained at pH 6.0 during the first 20 h and then shifted to pH 5.0 until the end of the fermentation. An optimal DHA concentration of 175.9 ± 6.7 g/L, with a production yield to glycerol of 0.87 ± 0.04 g/g, was obtained at 72 h of DO-stat fed-batch fermentation at 30°C in a 15 L fermenter.
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KEYWORD
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1,3-dihydroxyacetone, Gluconobacter oxydans, DO-stat fermentation, glycerol, fed-batch
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